The biological fermentation exhaust analyzer and biomass measurement system software can detect and analyze the CO2 and O2 concentration in the fermentation exhaust online for a long time, and calculate the relevant biomass parameters through the software, such as CO2 release rate CER, oxygen uptake rate OUR and respiratory quotient RQ. Through online detection of tail gas and parameter calculation, fermentation stages can be easily judged, which is of great significance to deeply understand and master the fermentation law of strains, study the fermentation process, reasonably adjust the ventilation and stirring speed, especially to guide the process operation. It can be widely used in various fermentation fields, especially in genetic engineering, biopharmaceutical and the application of the fermentation process using the flow process, which can greatly accelerate the development and industrialization of new products, stable production and improve the yield。